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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
19/06/2019 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H. |
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Afiliación : |
Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Ministério da Saúde, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil Silva, D.N. // Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Laboratory of Bacteriology and Health, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Saude Coletiva, Federal University of Bahia, Salvador, Brazil.; Instituto de Ciências da Saúde, Federal University of Bahia, Salvador, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil. |
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Título : |
Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391 |
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DOI : |
10.1371/journal.pone.0217391 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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Contenido : |
ABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. MenosABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected shee... Presentar Todo |
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Palabras claves : |
DISEASES OF SHEEP; LEPTOSPIRES; PLATAFORMA EN SALUD ANIMAL. |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES; LEPTOSPIROSIS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16705/1/pone.0217391.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/pdf/pone.0217391.pdf
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Marc : |
LEADER 03023naa a2200301 a 4500
001 1059867
005 2022-09-05
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0217391$2DOI
100 1 $aALMEIDA, D.S.
245 $aInvestigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
520 $aABSTRACT. The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.
650 $aENFERMEDADES DE LOS ANIMALES
650 $aLEPTOSPIROSIS
653 $aDISEASES OF SHEEP
653 $aLEPTOSPIRES
653 $aPLATAFORMA EN SALUD ANIMAL
700 1 $aPAZ, L.N.
700 1 $aDE OLIVEIRA, D.S.
700 1 $aRISTOW, P.
700 1 $aHAMOND, C.
700 1 $aCOSTA, F.
700 1 $aPORTELA, R.W.
700 1 $aESTRELA-LIMA, A.
700 1 $aPINNA, M.H.
773 $tPloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/04/2023 |
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Actualizado : |
27/04/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RAMOS, Z.; GARRICK, D.J.; BLAIR, H.T.; DE BARBIERI, I.; CIAPPESONI, G.; MONTOSSI, F.; KENYON, P.R. |
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Afiliación : |
ZULLY MARGOT RAMOS ALVEZ, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; DORIAN J. GARRICK, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; HUGH T. BLAIR, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAUL R. KENYON, School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand. |
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Título : |
Genetic and phenotypic relationships between ewe reproductive performance and wool and growth traits in Uruguayan Ultrafine Merino sheep. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Journal of Animal Science, 2023, Volume 101, skad071. OPEN ACCESS. https://doi.org/10.1093/jas/skad071 |
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ISSN : |
1525-3163 |
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DOI : |
10.1093/jas/skad071 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 13 July 2022; Accepted 06 March 2023; Published 07 March 2023; Corrected and typeset 14 April 2023. -- Corresponding author: z.ramosalvez@massey.ac.nz -- Issue Section: Animal Genetics and Genomics (https://academic.oup.com/jas/search-results?f_TocHeadingTitle=Animal+Genetics+and+Genomics ) -- Supplementary data are available at Journal of Animal Science online -- |
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Contenido : |
This study reports genetic parameters for yearling and adult wool and growth traits, and ewe reproductive performance. Data were sourced from an Uruguayan Merino flock involved in a long-term selection program focused on reduced fiber diameter (FD), and increased clean fleece weight (CFW) and live weight (LW). Pedigree and performance data from approximately 5,700 mixed-sex yearling lambs and 2,000 mixed-age ewes born between 1999 and 2019 were analyzed. The number of records ranged from 1,267 to 5,738 for yearling traits, and from 1,931 to 7,079 for ewe productive and reproductive performance. Data on yearling and adult wool traits, LW and body condition score (BCS), yearling eye muscle area (Y_EMA), and fat thickness (Y_FAT), and several reproduction traits were analyzed. The genetic relationships between FD and reproduction traits were not different from zero. © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science |
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Palabras claves : |
Body condition score; Correlations; Heritability; Merino; Reproduction; Wool. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://academic.oup.com/jas/article-pdf/doi/10.1093/jas/skad071/49893524/skad071.pdf
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Marc : |
LEADER 02293naa a2200301 a 4500
001 1064062
005 2023-04-27
008 2023 bl uuuu u00u1 u #d
022 $a1525-3163
024 7 $a10.1093/jas/skad071$2DOI
100 1 $aRAMOS, Z.
245 $aGenetic and phenotypic relationships between ewe reproductive performance and wool and growth traits in Uruguayan Ultrafine Merino sheep.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 13 July 2022; Accepted 06 March 2023; Published 07 March 2023; Corrected and typeset 14 April 2023. -- Corresponding author: z.ramosalvez@massey.ac.nz -- Issue Section: Animal Genetics and Genomics (https://academic.oup.com/jas/search-results?f_TocHeadingTitle=Animal+Genetics+and+Genomics ) -- Supplementary data are available at Journal of Animal Science online --
520 $aThis study reports genetic parameters for yearling and adult wool and growth traits, and ewe reproductive performance. Data were sourced from an Uruguayan Merino flock involved in a long-term selection program focused on reduced fiber diameter (FD), and increased clean fleece weight (CFW) and live weight (LW). Pedigree and performance data from approximately 5,700 mixed-sex yearling lambs and 2,000 mixed-age ewes born between 1999 and 2019 were analyzed. The number of records ranged from 1,267 to 5,738 for yearling traits, and from 1,931 to 7,079 for ewe productive and reproductive performance. Data on yearling and adult wool traits, LW and body condition score (BCS), yearling eye muscle area (Y_EMA), and fat thickness (Y_FAT), and several reproduction traits were analyzed. The genetic relationships between FD and reproduction traits were not different from zero. © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science
653 $aBody condition score
653 $aCorrelations
653 $aHeritability
653 $aMerino
653 $aReproduction
653 $aWool
700 1 $aGARRICK, D.J.
700 1 $aBLAIR, H.T.
700 1 $aDE BARBIERI, I.
700 1 $aCIAPPESONI, G.
700 1 $aMONTOSSI, F.
700 1 $aKENYON, P.R.
773 $tJournal of Animal Science, 2023, Volume 101, skad071. OPEN ACCESS. https://doi.org/10.1093/jas/skad071
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